STANDARD OPERATING PROCEDURE SOP-100.50 Common Equipment Room Laboratory Procedures … Remember the laboratory is a common area and always clean your work area for the safety and … STANDARD OPERATING PROCEDURE SOP-100.50 Common Equipment Room Laboratory Procedures Originator Wayne Johnson Company SEG Inc. Title Date 02/13/08 Approved By Department Title Date NOTICE: The information contained herein is confidential and proprietary information, and as such, cannot be copied or disclosed in whole or in part to others without …

Subject: Common Equipment Room Laboratory Procedures Revision 02 Page Page 4 of 8 Doc. No. SOP 100.50 NOTICE: The information contained herein is confidential and proprietary information, and as such, cannot be copied or disclosed in whole or in part to others without the prior consent of the San Jose BioCenter SOP Number 100.50 Page 4 of 8 Rev. 02 etc. Hazardous liquid chemicals must be stored below eye level. Work areas and floors will be kept clear of and not used as excessive storage space. Common labs such as the Bacterial Culture Room or Service Alcove are shared by all tenants of the BioCenter. Keep these rooms accessible and clean. Do not use these areas as long term storage space. 5.5 Laboratory Hygiene- Wash hands upon exiting the laboratory. At a minimum, always wear a laboratory coat and gloves suitable for the tasks being conducted when in the laboratory. Cover legs and feet (no sandals, open-toed shoes or shorts), and confine loose clothing and long hair. Avoid touching exposed skin with laboratory coat or gloves. Always wear appropriate PPE for the experiment being performed. Avoid contaminating high use items such as door handles, faucets and light switches. Assume that the laboratory is a high risk environment. Always clean your work area prior to beginning work. Remember the laboratory is a common area and always clean your work area for the safety and protection of other laboratory users. 5.6 Equipment- Use proper equipment that is in good condition. For example, never use chipped or cracked glassware. Shield pressurized or vacuum apparatus and safeguard against bumping or overheating. When hazardous experiments are to be conducted, the person conducting the experiment is responsible for verifying that the experiment is conducted in an appropriate and safe manner using the correct equipment. All users must sign the log book before using the equipment. In the event equipment is not working to specifications, it is user responsibility to notify SJBC staff immediately. 5.7 Experimental Hazard Assessment- review experimental protocols and research of new techniques that consider the hazards and quantities of waste produced. Wherever possible, experiments shall be conducted in exhausted enclosures. Destruction procedures shall be used as the final step in experiments; e.g., neutralization of corrosive wastes that do not contain heavy metals or addition of a known biocide for experimental cells in any waste to be disposed. 5.8 Disposal of Hazardous Materials- In no circumstance will Biohazard or Chemical waste be dumped into sanitary or laboratory sinks. The experimental procedure shall account for chemical, biological and hazardous byproduct disposal. Flammable and Corrosive Waste and items contaminated with hazardous waste will be disposed of in appropriate and labeled waste containers. In no case shall laboratory waste be disposed of in general waste containers. This includes disposal of empty chemical containers (regardless of rinsing or washing). 5.9 Chemical spills and accident response- In the event of a chemical spill notify SJBC staff immediately. If the event occurs after regular hours contact

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